Identication of Variance Moleculer Genotype Commercial Oil Palm (Elaeis guineensis Jacq.) based on Random Amplified Polymorpism DNA (RAPD) Markers

Arnen Pasaribu, Lollie Agustina P.Putri, Dwi Suryanto


Abstract —Commercial oil palm is one of plant genotype have a inbreeding so that it will be genetic depression.  Genetic depression can cause difficulties to identification of genetic trait in palm oil, so that identification of moleculer variance is necessary to know the genetic potential of commercial palm oil to obtain initial information about genetic diversity. The objectives of this study was to find genetic diversity in commercial oil palm (E. guineensis Jacq.) by using five RAPD primers (OPC-12, OPH-12, OPC-7, OPI-20 and OPD-16). The results of this study showed that Polymorpism Information Content (PIC) was 0.338.  PCoA analysis showed that the moleculer varian was 43,72% and according to profil radial neighbour joining tree (NJtree) showed that three main groups.


KeywordsGenetic Variation,Oil Palm , RAPD

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D. Arias, C. Montoya, L. Rey, and H. Romero “Genetic similiarity among commercial oil palm materials based on microsatellite markers”. Agronomia Colombiana . Vol. 30 (2). pp. 188-195. April. 2012.

M. N. Okoye, M.I. Uguru, C. Bkome, R. Sigh, C. O. Okwuagwu. “Assesment of Genetic diversity of NIFOR oil palm breeding parent genotypes using microsatellite markers”. American Journal of Plant Science, Vol. 7. pp.218-237. January. 2016

L.X.Zhou, Y.Xiao, W.Xia and Y.D. Yang. “Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on sepecies-spesific simple sequence repeats markers”.Genetic and Molecular Research. Vol. 14 (4). pp. 16247-162454. December. 2015.

N.Luyindula, N. Mantantu., F.Dumortier and R.H.V. Corley. “Effect of inbreeding on growth and yield of oil palm”. Eupthyca. International Journal of Plant Breeding. Vol. 143. Pp.9-17. January.2005

[25] G. Acquaah, Principles of plant genetics and breeding. Blacwell publishing, 2007, pp. 282-291.

M. Sohrabi, M. Y. Rafii, M.M.Hanafi and Md. A. Latif. “Genetic divergence of Malaysian upland rice revealed by microsatellite markers”. Plant Omic Journal. Vol. 6(3). pp. 175-182. 2013.

T. Khan, O. H. Reza, A. Khan, S. Haque, S.Islam1, and B. Khan. “Genetic Diversity Analysis of Cowpea by RAPD Markers”. International Journal of Innovation and Applied Studies. Vol. 10. pp. 459-465. Feb. 2015.

J.C. Millwe and S.D.Tanksley, “RFLP analysis of phylogenetic relationship and genetic variation in the genus Lycopersicon ”. Theor. Appl. Genet. Vol. 80, pp. 437-448, 1990.

D.K Garcia and J. Benzie, “RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs”, Aquaculture. Vol. 130, pp. 137-144, 1995.

B. Neekhra, A. Mansoori, S. Verma, R. K. Koiri and S.K Jain. “RAPD-PCR Based Biomarker Study in Fish Species (Family: Cyprinidae) of Madhya Pradesh, India” Austin J Mol & Cell Biol. Vol. 1. pp. 1-6, December, 2014.

M. Kantii, K. Anjani, Betha, N.I. and K.Viveknanda,“Agro-morphological and molecular diversity in Castor (Ricinus communis L.) germplasm collected from andaman and nicobar islands, India”, Czech J. Genet. Plant Breed, Vol. 3, pp. 96–109, Oktober, 2015.

K. Weising, H. Nybom, K. Wolf and G. Kahl, DNA Fingerprinting in Plants Principles, Methods and Application Second Edition.. CRC Press Taylor & Franchis, 2005, pp. 24-73.

D.K. Sathish and C Mohankumar, “RAPD markers for identifying oil palm (Elaeis guineensis Jacq.) parental varieties (dura & pisifera) and the hybrid tenera ”, Indian Journal of Biotechnology. Vol 6, pp 354-358, July. 2007.

Kapteyn, J., Simon, J.E.. The use of RAPDs for assessment of identity, diversity, and quality of Echinacea. In: Trends in new crops and new uses . pp. 509-513. ASHS Press, Alexandria, VA. 2002.

H.N.Lakhani, S.V.Patel, N.P.Bodar and B.A.Golakiya, “RAPD analysis of genetic diversity of castor bean (Ricinus communis L.)”, Int.J.Curr.Microbiol. App.Sci Vol. 4, pp. 696-703, 2015.

Z. Mei, C. Zhang, Md. A. Khan, Y. Zhu, M. Tania, P. Luo and J.Fu. “Eficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationship in Angelica sinensis (oliv.)” diels vairieties. Electronic Journal of Biothechnology hosted by Elsevier. pp. 96-102. January. 2015

J.H.C.Sanchez. K. Remarchuk and K.U. Sena., “Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous”, Plant moleculer biology. Vol. 24, pp. 161-167, 2006.

I. R. Ruiz, J. Dendauw, E.V. Bockstaele, A. Depicker and M.D. Loose. “AFLP markers reveal high polymorphic rates in ryegrasses (Lollium spp.)”, Moleceler. Breeing. Vol. 6. pp. 125-134., 2000.

J. D. Riek, E. Calsyn, E, Everaert, E.V. Bockstaele E, M.D. :Loose, AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theor Appl Genet. Vol.103. pp. 1254- 1265. 2001.

Perreira and J. Jacque. (July, 2014). Tutorial Softwere DARwin (Dissimiliarity Analysis Representation for Windows). DARwin software Available :

Noruya,S. and Masatoshi, N., “The Neighbor-Joining method: a new method for reconstructing phylogenetic trees”, Molecular Biology and Evolution. Vol. 4, pp. 406-425, 1987.

K.V. Bhat, P.P. Babrekar and S. Lakhanpul, “Study of genetic diversity in indian and exotic sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers (Periodical style)”, Euphytica, Vol. 110, pp. 21-33. October, 1999.

D.K. Sathish and C. Mohankumar, “RAPD markers for identifying oil palm (Elaeis guineensis Jacq.) parental varieties (dura & pisifera) and the hybrid tenera (Periodical style)”, Indian Journal of Biotechnology. Vol 6, pp 354-358, July. 2007.

V.D. Odenore, C.R. Eke, O. Asemota and H.O. Shittu. Determination of phylogenetic relationship among oil palm (Elaeis guineensis) Varieties with RAPD. European International Journal of Sciencve and Technology. Vol. 4. (2). February. 2015.



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